Altered human gut dendritic cell properties in ulcerative colitis are reversed by Lactobacillus plantarum extracellular encrypted peptide STp

Research output: Contribution to journalArticle

  • External authors:
  • Hafid O Al-Hassi
  • Borja Sanchez
  • Nicholas R English
  • Simon T C Peake
  • Jonathan Landy
  • Ripple Man
  • Maria Urdaci
  • Ailsa L Hart
  • Luis Fernandez-Salazar
  • Gui Han Lee
  • Jose A Garrote
  • Eduardo Arranz
  • Abelardo Margolles
  • Andrew J Stagg
  • Stella C Knight
  • David Bernardo

Abstract

SCOPE: The human/microbiota cross-talk is partially mediated by bacteria-derived peptides like Serine-Threonine peptide (STp), which is resistant to gut proteolysis, is found in the human healthy colon and induces regulatory properties on gut dendritic cells (DCs); here we characterized human gut DC in ulcerative colitis (UC) patients and studied the effect of STp on their properties.

METHODS AND RESULTS: Human colonic DC from healthy controls and UC patients were isolated, conditioned for 24 h +/- STp and characterized by flow cytometry, immunohistochemistry, and electron microscopy. Expression of immature DC markers DC-SIGN and ILT3, and Toll-like receptors were increased on gut UC-DC. Langerin (involved in phagocytosis), lymph node homing marker CCR7, and activation markers CD40/CD80/CD86 were decreased in UC. Gut DC had restricted stimulatory capacity for T-cells in UC. Conditioning of DC with STp in vitro reduced Toll-like receptor expression, increased CD40 and CD80 expression, and restored their stimulatory capacity.

CONCLUSION: Colonic DCs display an abnormal immature phenotype in UC, which was partially restored following STp treatment. Bacteria-derived metabolites, like STp, seem to have a role in gut homeostasis that is missing in UC so they might lead a new era of probiotic products setting the basis for nondrug dietary therapy in inflammatory bowel disease.

Bibliographical metadata

Original languageEnglish
Pages (from-to)1132-43
Number of pages12
JournalMolecular nutrition & food research
Volume58
Issue number5
DOIs
Publication statusPublished - May 2014

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