Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis.Citation formats

  • External authors:
  • Andrew P Breidenbach
  • Lindsey Aschbacher-Smith
  • Yinhui Lu
  • Nathaniel A Dyment
  • Chia-Feng Liu
  • Han Liu
  • Chris Wylie
  • Marepalli Rao
  • Jason T Shearn
  • David W Rowe
  • Rulang Jiang
  • David L Butler

Standard

Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis. / Breidenbach, Andrew P; Aschbacher-Smith, Lindsey; Lu, Yinhui; Dyment, Nathaniel A; Liu, Chia-Feng; Liu, Han; Wylie, Chris; Rao, Marepalli; Shearn, Jason T; Rowe, David W; Kadler, Karl E; Jiang, Rulang; Butler, David L.

In: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 33, No. 8, 08.2015.

Research output: Contribution to journalArticle

Harvard

Breidenbach, AP, Aschbacher-Smith, L, Lu, Y, Dyment, NA, Liu, C-F, Liu, H, Wylie, C, Rao, M, Shearn, JT, Rowe, DW, Kadler, KE, Jiang, R & Butler, DL 2015, 'Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis.' Journal of orthopaedic research : official publication of the Orthopaedic Research Society, vol. 33, no. 8. https://doi.org/10.1002/jor.22899, https://doi.org/10.1002/jor.22899

APA

Breidenbach, A. P., Aschbacher-Smith, L., Lu, Y., Dyment, N. A., Liu, C-F., Liu, H., ... Butler, D. L. (2015). Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 33(8). https://doi.org/10.1002/jor.22899, https://doi.org/10.1002/jor.22899

Vancouver

Breidenbach AP, Aschbacher-Smith L, Lu Y, Dyment NA, Liu C-F, Liu H et al. Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 2015 Aug;33(8). https://doi.org/10.1002/jor.22899, https://doi.org/10.1002/jor.22899

Author

Breidenbach, Andrew P ; Aschbacher-Smith, Lindsey ; Lu, Yinhui ; Dyment, Nathaniel A ; Liu, Chia-Feng ; Liu, Han ; Wylie, Chris ; Rao, Marepalli ; Shearn, Jason T ; Rowe, David W ; Kadler, Karl E ; Jiang, Rulang ; Butler, David L. / Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis. In: Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 2015 ; Vol. 33, No. 8.

Bibtex

@article{99753ed547774caeaf57b58baca5f537,
title = "Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis.",
abstract = "Restoring the native structure of the tendon enthesis, where collagen fibers of the midsubstance are integrated within a fibrocartilaginous structure, is problematic following injury. As current surgical methods fail to restore this region adequately, engineers, biologists, and clinicians are working to understand how this structure forms as a prerequisite to improving repair outcomes. We recently reported on the role of Indian hedgehog (Ihh), a novel enthesis marker, in regulating early postnatal enthesis formation. Here, we investigate how inactivating the Hh pathway in tendon cells affects adult (12-week) murine patellar tendon (PT) enthesis mechanics, fibrocartilage morphology, and collagen fiber organization. We show that ablating Hh signaling resulted in greater than 100{\%} increased failure insertion strain (0.10 v. 0.05 mm/mm, p",
keywords = "enthesis, fibrocartilage, hedgehog, mineralization, serial block face-scanning electron microscopy, tendon mechanics",
author = "Breidenbach, {Andrew P} and Lindsey Aschbacher-Smith and Yinhui Lu and Dyment, {Nathaniel A} and Chia-Feng Liu and Han Liu and Chris Wylie and Marepalli Rao and Shearn, {Jason T} and Rowe, {David W} and Kadler, {Karl E} and Rulang Jiang and Butler, {David L}",
note = "091840/Z/10/Z, Wellcome Trust, United KingdomAR056943-05, NIAMS NIH HHS, United StatesDE013681, NIDCR NIH HHS, United StatesDE021989-02, NIDCR NIH HHS, United StatesR01 AR056943, NIAMS NIH HHS, United States",
year = "2015",
month = "8",
doi = "10.1002/jor.22899",
language = "English",
volume = "33",
journal = "Journal of orthopaedic research : official publication of the Orthopaedic Research Society",
issn = "1554-527X",
publisher = "John Wiley & Sons Ltd",
number = "8",

}

RIS

TY - JOUR

T1 - Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis.

AU - Breidenbach, Andrew P

AU - Aschbacher-Smith, Lindsey

AU - Lu, Yinhui

AU - Dyment, Nathaniel A

AU - Liu, Chia-Feng

AU - Liu, Han

AU - Wylie, Chris

AU - Rao, Marepalli

AU - Shearn, Jason T

AU - Rowe, David W

AU - Kadler, Karl E

AU - Jiang, Rulang

AU - Butler, David L

N1 - 091840/Z/10/Z, Wellcome Trust, United KingdomAR056943-05, NIAMS NIH HHS, United StatesDE013681, NIDCR NIH HHS, United StatesDE021989-02, NIDCR NIH HHS, United StatesR01 AR056943, NIAMS NIH HHS, United States

PY - 2015/8

Y1 - 2015/8

N2 - Restoring the native structure of the tendon enthesis, where collagen fibers of the midsubstance are integrated within a fibrocartilaginous structure, is problematic following injury. As current surgical methods fail to restore this region adequately, engineers, biologists, and clinicians are working to understand how this structure forms as a prerequisite to improving repair outcomes. We recently reported on the role of Indian hedgehog (Ihh), a novel enthesis marker, in regulating early postnatal enthesis formation. Here, we investigate how inactivating the Hh pathway in tendon cells affects adult (12-week) murine patellar tendon (PT) enthesis mechanics, fibrocartilage morphology, and collagen fiber organization. We show that ablating Hh signaling resulted in greater than 100% increased failure insertion strain (0.10 v. 0.05 mm/mm, p

AB - Restoring the native structure of the tendon enthesis, where collagen fibers of the midsubstance are integrated within a fibrocartilaginous structure, is problematic following injury. As current surgical methods fail to restore this region adequately, engineers, biologists, and clinicians are working to understand how this structure forms as a prerequisite to improving repair outcomes. We recently reported on the role of Indian hedgehog (Ihh), a novel enthesis marker, in regulating early postnatal enthesis formation. Here, we investigate how inactivating the Hh pathway in tendon cells affects adult (12-week) murine patellar tendon (PT) enthesis mechanics, fibrocartilage morphology, and collagen fiber organization. We show that ablating Hh signaling resulted in greater than 100% increased failure insertion strain (0.10 v. 0.05 mm/mm, p

KW - enthesis

KW - fibrocartilage

KW - hedgehog

KW - mineralization

KW - serial block face-scanning electron microscopy

KW - tendon mechanics

U2 - 10.1002/jor.22899

DO - 10.1002/jor.22899

M3 - Article

VL - 33

JO - Journal of orthopaedic research : official publication of the Orthopaedic Research Society

JF - Journal of orthopaedic research : official publication of the Orthopaedic Research Society

SN - 1554-527X

IS - 8

ER -