Ablating hedgehog signaling in tenocytes during development impairs biomechanics and matrix organization of the adult murine patellar tendon enthesis.

Research output: Contribution to journalArticle

  • External authors:
  • Andrew P Breidenbach
  • Lindsey Aschbacher-Smith
  • Yinhui Lu
  • Nathaniel A Dyment
  • Chia-Feng Liu
  • Han Liu
  • Chris Wylie
  • Marepalli Rao
  • Jason T Shearn
  • David W Rowe
  • Rulang Jiang
  • David L Butler

Abstract

Restoring the native structure of the tendon enthesis, where collagen fibers of the midsubstance are integrated within a fibrocartilaginous structure, is problematic following injury. As current surgical methods fail to restore this region adequately, engineers, biologists, and clinicians are working to understand how this structure forms as a prerequisite to improving repair outcomes. We recently reported on the role of Indian hedgehog (Ihh), a novel enthesis marker, in regulating early postnatal enthesis formation. Here, we investigate how inactivating the Hh pathway in tendon cells affects adult (12-week) murine patellar tendon (PT) enthesis mechanics, fibrocartilage morphology, and collagen fiber organization. We show that ablating Hh signaling resulted in greater than 100% increased failure insertion strain (0.10 v. 0.05 mm/mm, p

Bibliographical metadata

Original languageEnglish
JournalJournal of orthopaedic research : official publication of the Orthopaedic Research Society
Volume33
Issue number8
DOIs
Publication statusPublished - Aug 2015