Ability to undergo apoptosis does not correlate with the intrinsic radiosensitivity (SF2) of human cervix tumor cell linesCitation formats

Standard

Ability to undergo apoptosis does not correlate with the intrinsic radiosensitivity (SF2) of human cervix tumor cell lines. / Sheridan, Mary T.; West, Catharine M L.

In: International Journal of Radiation Oncology Biology Physics, Vol. 50, No. 2, 01.06.2001, p. 503-509.

Research output: Contribution to journalArticlepeer-review

Harvard

APA

Vancouver

Author

Sheridan, Mary T. ; West, Catharine M L. / Ability to undergo apoptosis does not correlate with the intrinsic radiosensitivity (SF2) of human cervix tumor cell lines. In: International Journal of Radiation Oncology Biology Physics. 2001 ; Vol. 50, No. 2. pp. 503-509.

Bibtex

@article{60e7bd3df9ed4e4d85145bb7afc66f29,
title = "Ability to undergo apoptosis does not correlate with the intrinsic radiosensitivity (SF2) of human cervix tumor cell lines",
abstract = "Purpose: To investigate the relationship between radiation-induced apoptosis and clonogenic cell kill in 9 cervical cancer cell lines. Methods and Materials: Cells were irradiated with 0, 2, 8, and 30 Gy. The level of apoptosis was evaluated using flow cytometry (Annexin-V binding), light microscropy (morphology), gel electrophoresis (DNA ladder formation), and TUNEL assay. Cell survival was measured using a clonogenic assay. Results: Of the 9 cervical carcinoma cell lines analyzed, 3 underwent radiation-induced apoptosis: CaSki, HT3, and 778. The levels of apoptosis, obtained 72 h after a dose of 30 Gy, were 49%, 28%, and 26%, respectively. All cell lines exhibited some level of background apoptosis measured by Annexin-V binding (mean = 2.6% ± 0.8; range, 0.2-6.9%) that correlated with the level of radiation-induced apoptosis (r = 0.92, p = 0.001). In 6 of the 9 lines, necrosis was the dominant form of cell death. A significant inverse relationship was found between the level of radiation-induced apoptosis and necrosis after 30 Gy (r = -0.87, p = 0.002). No relationship was found between radiation-induced apoptosis and intrinsic radiosensitivity measured, using a clonogenic assay, as surviving fraction at 2 Gy (SF2). Conclusion: Cervical carcinoma cells do not readily undergo radiation-induced apoptosis in vitro. There is no relationship between ability to undergo apoptosis and intrinsic radiosensitivity measured using a clonogenic assay. Copyright {\textcopyright} 2001 Elsevier Science Inc.",
keywords = "Apoptosis, Cervix, Clonogenicity, Radiation, Survival",
author = "Sheridan, {Mary T.} and West, {Catharine M L}",
year = "2001",
month = jun,
day = "1",
doi = "10.1016/S0360-3016(01)01496-1",
language = "English",
volume = "50",
pages = "503--509",
journal = "International Journal of Radiation: Oncology - Biology - Physics",
issn = "0360-3016",
publisher = "Elsevier BV",
number = "2",

}

RIS

TY - JOUR

T1 - Ability to undergo apoptosis does not correlate with the intrinsic radiosensitivity (SF2) of human cervix tumor cell lines

AU - Sheridan, Mary T.

AU - West, Catharine M L

PY - 2001/6/1

Y1 - 2001/6/1

N2 - Purpose: To investigate the relationship between radiation-induced apoptosis and clonogenic cell kill in 9 cervical cancer cell lines. Methods and Materials: Cells were irradiated with 0, 2, 8, and 30 Gy. The level of apoptosis was evaluated using flow cytometry (Annexin-V binding), light microscropy (morphology), gel electrophoresis (DNA ladder formation), and TUNEL assay. Cell survival was measured using a clonogenic assay. Results: Of the 9 cervical carcinoma cell lines analyzed, 3 underwent radiation-induced apoptosis: CaSki, HT3, and 778. The levels of apoptosis, obtained 72 h after a dose of 30 Gy, were 49%, 28%, and 26%, respectively. All cell lines exhibited some level of background apoptosis measured by Annexin-V binding (mean = 2.6% ± 0.8; range, 0.2-6.9%) that correlated with the level of radiation-induced apoptosis (r = 0.92, p = 0.001). In 6 of the 9 lines, necrosis was the dominant form of cell death. A significant inverse relationship was found between the level of radiation-induced apoptosis and necrosis after 30 Gy (r = -0.87, p = 0.002). No relationship was found between radiation-induced apoptosis and intrinsic radiosensitivity measured, using a clonogenic assay, as surviving fraction at 2 Gy (SF2). Conclusion: Cervical carcinoma cells do not readily undergo radiation-induced apoptosis in vitro. There is no relationship between ability to undergo apoptosis and intrinsic radiosensitivity measured using a clonogenic assay. Copyright © 2001 Elsevier Science Inc.

AB - Purpose: To investigate the relationship between radiation-induced apoptosis and clonogenic cell kill in 9 cervical cancer cell lines. Methods and Materials: Cells were irradiated with 0, 2, 8, and 30 Gy. The level of apoptosis was evaluated using flow cytometry (Annexin-V binding), light microscropy (morphology), gel electrophoresis (DNA ladder formation), and TUNEL assay. Cell survival was measured using a clonogenic assay. Results: Of the 9 cervical carcinoma cell lines analyzed, 3 underwent radiation-induced apoptosis: CaSki, HT3, and 778. The levels of apoptosis, obtained 72 h after a dose of 30 Gy, were 49%, 28%, and 26%, respectively. All cell lines exhibited some level of background apoptosis measured by Annexin-V binding (mean = 2.6% ± 0.8; range, 0.2-6.9%) that correlated with the level of radiation-induced apoptosis (r = 0.92, p = 0.001). In 6 of the 9 lines, necrosis was the dominant form of cell death. A significant inverse relationship was found between the level of radiation-induced apoptosis and necrosis after 30 Gy (r = -0.87, p = 0.002). No relationship was found between radiation-induced apoptosis and intrinsic radiosensitivity measured, using a clonogenic assay, as surviving fraction at 2 Gy (SF2). Conclusion: Cervical carcinoma cells do not readily undergo radiation-induced apoptosis in vitro. There is no relationship between ability to undergo apoptosis and intrinsic radiosensitivity measured using a clonogenic assay. Copyright © 2001 Elsevier Science Inc.

KW - Apoptosis

KW - Cervix

KW - Clonogenicity

KW - Radiation

KW - Survival

U2 - 10.1016/S0360-3016(01)01496-1

DO - 10.1016/S0360-3016(01)01496-1

M3 - Article

VL - 50

SP - 503

EP - 509

JO - International Journal of Radiation: Oncology - Biology - Physics

JF - International Journal of Radiation: Oncology - Biology - Physics

SN - 0360-3016

IS - 2

ER -