A 'Plug and Play' Platform for the Production of Diverse Monoterpene Hydrocarbon Scaffolds in Escherichia coli

Research output: Contribution to journalArticle

  • External authors:
  • Nicole Leferink
  • Ziga Zebec
  • Helen Toogood

Abstract

The terpenoids constitute one of the largest and most diverse classes of natural compounds with applications as pharmaceuticals, flavorings and fragrances, pesticides and biofuels. Synthetic biology is ideally placed to create new routes to this chemical diversity and facilitation of new compound discovery. The C10 monoterpenoids display a huge structural diversity produced from a single substrate, geranyl diphosphate, by a family of monoterpene cyclases and synthases (mTC/S). Here we employ a library of mTC/S in a single ‘plug and play’ platform system for the production of over 30 different monoterpenoids in Escherichia coli by fermentation on glucose. These products include several compounds never before produced in engineered microbes demonstrating the power of this approach to rapidly create routes to structural diversity.

Bibliographical metadata

Original languageEnglish
Pages (from-to)1893-1896
Number of pages4
JournalChemistrySelect
Volume1
Issue number9
Early online date21 Jun 2016
DOIs
StatePublished - Jun 2016

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