A novel method for sampling the microbiota from the oral mucosaCitation formats

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A novel method for sampling the microbiota from the oral mucosa. / Rusanen, Peter; Siikala, Emilia; Uittamo, Johanna; Richardson, Malcolm; Rautemaa-Richardson , Riina.

In: Clinical Oral Investigations, Vol. 13, No. 2, 2009, p. 243-246.

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Rusanen, P, Siikala, E, Uittamo, J, Richardson, M & Rautemaa-Richardson , R 2009, 'A novel method for sampling the microbiota from the oral mucosa', Clinical Oral Investigations, vol. 13, no. 2, pp. 243-246. https://doi.org/10.1007/s00784-008-0225-5

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Rusanen, Peter ; Siikala, Emilia ; Uittamo, Johanna ; Richardson, Malcolm ; Rautemaa-Richardson , Riina. / A novel method for sampling the microbiota from the oral mucosa. In: Clinical Oral Investigations. 2009 ; Vol. 13, No. 2. pp. 243-246.

Bibtex

@article{4c53fa90630d4141931e65a9af0e937c,
title = "A novel method for sampling the microbiota from the oral mucosa",
abstract = "The purpose of this study was to develop a site-specific sampling method that could give representative and quantitative results for defined areas of the oral mucosa and would be easy to use. Two site-specific sampling methods (swab and filter paper imprint) were compared. The filter paper sampling method was developed for this study. Samples were collected from 14 volunteers. All samples were cultured under aerobic and anaerobic conditions. The number of viable bacteria and yeasts was determined and expressed per unit area. The filter paper recovered a significantly higher number of colony types of bacteria compared to the swab sample. Both collected a large number and variety of different oral microbes. The filter paper sampling method could be the optimal technique for quantitative site-specific oral mucosal samples and is highly suitable for both culture-based and non-culture-based identification of oral microbes. {\circledC} Springer-Verlag 2008.",
keywords = "Oral bacteria, Oral mucosa, Sampling",
author = "Peter Rusanen and Emilia Siikala and Johanna Uittamo and Malcolm Richardson and Riina Rautemaa-Richardson",
year = "2009",
doi = "10.1007/s00784-008-0225-5",
language = "English",
volume = "13",
pages = "243--246",
journal = "Clinical Oral Investigations",
issn = "1432-6981",
publisher = "Springer Nature",
number = "2",

}

RIS

TY - JOUR

T1 - A novel method for sampling the microbiota from the oral mucosa

AU - Rusanen, Peter

AU - Siikala, Emilia

AU - Uittamo, Johanna

AU - Richardson, Malcolm

AU - Rautemaa-Richardson , Riina

PY - 2009

Y1 - 2009

N2 - The purpose of this study was to develop a site-specific sampling method that could give representative and quantitative results for defined areas of the oral mucosa and would be easy to use. Two site-specific sampling methods (swab and filter paper imprint) were compared. The filter paper sampling method was developed for this study. Samples were collected from 14 volunteers. All samples were cultured under aerobic and anaerobic conditions. The number of viable bacteria and yeasts was determined and expressed per unit area. The filter paper recovered a significantly higher number of colony types of bacteria compared to the swab sample. Both collected a large number and variety of different oral microbes. The filter paper sampling method could be the optimal technique for quantitative site-specific oral mucosal samples and is highly suitable for both culture-based and non-culture-based identification of oral microbes. © Springer-Verlag 2008.

AB - The purpose of this study was to develop a site-specific sampling method that could give representative and quantitative results for defined areas of the oral mucosa and would be easy to use. Two site-specific sampling methods (swab and filter paper imprint) were compared. The filter paper sampling method was developed for this study. Samples were collected from 14 volunteers. All samples were cultured under aerobic and anaerobic conditions. The number of viable bacteria and yeasts was determined and expressed per unit area. The filter paper recovered a significantly higher number of colony types of bacteria compared to the swab sample. Both collected a large number and variety of different oral microbes. The filter paper sampling method could be the optimal technique for quantitative site-specific oral mucosal samples and is highly suitable for both culture-based and non-culture-based identification of oral microbes. © Springer-Verlag 2008.

KW - Oral bacteria

KW - Oral mucosa

KW - Sampling

U2 - 10.1007/s00784-008-0225-5

DO - 10.1007/s00784-008-0225-5

M3 - Article

VL - 13

SP - 243

EP - 246

JO - Clinical Oral Investigations

JF - Clinical Oral Investigations

SN - 1432-6981

IS - 2

ER -