A Nanostructured Aptamer-Functionalised Black Phosphorus Sensing Platform for Label-Free Detection of Myoglobin, a Cardiovascular Disease Biomarker

Research output: Contribution to journalArticle

  • External authors:
  • Vinod Kumar
  • Jack Brent
  • Munish Shorie
  • Harmanjit Kaur
  • Gaganpreet Chadha
  • Edward Lewis
  • Aidan Rooney
  • Lan Nguyen
  • Xiang Li Zhong
  • Paul Mcnaughter
  • Aleksander Tedstone
  • Nicky Savjani
  • Christopher Muryn
  • Paul O'Brien
  • Ashok K. Ganguli
  • Priyanka Sabherwal

Abstract

We report the electrochemical detection of the redox active cardiac biomarker myoglobin (Mb) using aptamer-functionalized black phosphorus nanostructured electrodes by measuring direct electron transfer. The as-synthesized few-layer black phosphorus nanosheets have been functionalized with poly-l-lysine (PLL) to facilitate binding with generated anti-Mb DNA aptamers on nanostructured electrodes. This aptasensor platform has a record-low detection limit (∼0.524 pg mL–1) and sensitivity (36 μA pg–1 mL cm–2) toward Mb with a dynamic response range from 1 pg mL–1 to 16 μg mL–1 for Mb in serum samples. This strategy opens up avenues to bedside technologies for multiplexed diagnosis of cardiovascular diseases in complex human samples.

Bibliographical metadata

Original languageEnglish
Pages (from-to)22860–22868
JournalACS Applied Materials and Interfaces
Volume8
Issue number35
DOIs
StatePublished - 10 Aug 2016

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