A limitation of the method for siRNA delivery into primary human cytotrophoblast cellsCitation formats

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A limitation of the method for siRNA delivery into primary human cytotrophoblast cells. / Desforges, Michelle; Westwood, M.

In: Placenta, Vol. 32, No. 2, 02.2011, p. 192-194.

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@article{0fa9c4977fa843ecb884b15e139c5f13,
title = "A limitation of the method for siRNA delivery into primary human cytotrophoblast cells",
abstract = "Transfection using cationic lipid reagents such as DharmaFECT2 is an efficient tool for introducing siRNA into primary human cytotrophoblast cells. However, we now report a limitation to the use of this method as the concentration of DharamFECT2 needed for effective siRNA delivery causes ligand-independent activation of the insulin/insulin-like growth factor (IGF) receptor and consequently, functional resistance to cytotrophoblast stimulation by these two hormones. We therefore advise researchers against the use of this method of transfection when investigating the mechanisms by which insulin/IGF, and potentially other hormones that exert their effects through kinase signalling molecules, influence cytotrophoblast cell function. {\circledC} 2010 Elsevier Ltd. All rights reserved.",
keywords = "DharmaFECT, IGF-I, Insulin, Insulin receptor, Transfection",
author = "Michelle Desforges and M. Westwood",
year = "2011",
month = "2",
doi = "10.1016/j.placenta.2010.11.013",
language = "English",
volume = "32",
pages = "192--194",
journal = "Placenta",
issn = "0143-4004",
publisher = "Elsevier BV",
number = "2",

}

RIS

TY - JOUR

T1 - A limitation of the method for siRNA delivery into primary human cytotrophoblast cells

AU - Desforges, Michelle

AU - Westwood, M.

PY - 2011/2

Y1 - 2011/2

N2 - Transfection using cationic lipid reagents such as DharmaFECT2 is an efficient tool for introducing siRNA into primary human cytotrophoblast cells. However, we now report a limitation to the use of this method as the concentration of DharamFECT2 needed for effective siRNA delivery causes ligand-independent activation of the insulin/insulin-like growth factor (IGF) receptor and consequently, functional resistance to cytotrophoblast stimulation by these two hormones. We therefore advise researchers against the use of this method of transfection when investigating the mechanisms by which insulin/IGF, and potentially other hormones that exert their effects through kinase signalling molecules, influence cytotrophoblast cell function. © 2010 Elsevier Ltd. All rights reserved.

AB - Transfection using cationic lipid reagents such as DharmaFECT2 is an efficient tool for introducing siRNA into primary human cytotrophoblast cells. However, we now report a limitation to the use of this method as the concentration of DharamFECT2 needed for effective siRNA delivery causes ligand-independent activation of the insulin/insulin-like growth factor (IGF) receptor and consequently, functional resistance to cytotrophoblast stimulation by these two hormones. We therefore advise researchers against the use of this method of transfection when investigating the mechanisms by which insulin/IGF, and potentially other hormones that exert their effects through kinase signalling molecules, influence cytotrophoblast cell function. © 2010 Elsevier Ltd. All rights reserved.

KW - DharmaFECT

KW - IGF-I

KW - Insulin

KW - Insulin receptor

KW - Transfection

U2 - 10.1016/j.placenta.2010.11.013

DO - 10.1016/j.placenta.2010.11.013

M3 - Article

VL - 32

SP - 192

EP - 194

JO - Placenta

JF - Placenta

SN - 0143-4004

IS - 2

ER -