A Label-free Selected Reaction Monitoring Workflow Identifies a Subset of Pregnancy Specific Glycoproteins as Potential Predictive Markers of Early-onset Pre-eclampsiaCitation formats

  • External authors:
  • Richard T. Blankley
  • Christal Fisher
  • Robyn North
  • Philip Baker
  • Michael Walker
  • Andrew Williamson
  • Wanchang Lin
  • Lesley McCowan
  • Claire T. Roberts

Standard

A Label-free Selected Reaction Monitoring Workflow Identifies a Subset of Pregnancy Specific Glycoproteins as Potential Predictive Markers of Early-onset Pre-eclampsia. / Blankley, Richard T.; Fisher, Christal; Westwood, Melissa; North, Robyn; Baker, Philip; Walker, Michael; Williamson, Andrew; Whetton, Anthony D.; Lin, Wanchang; McCowan, Lesley; Roberts, Claire T.; Cooper, Garth J S; Unwin, Richard D.; Myers, Jenny E.

In: Molecular and Cellular Proteomics, Vol. 12, No. 11, 11.2013, p. 3148-3159.

Research output: Contribution to journalArticle

Harvard

Blankley, RT, Fisher, C, Westwood, M, North, R, Baker, P, Walker, M, Williamson, A, Whetton, AD, Lin, W, McCowan, L, Roberts, CT, Cooper, GJS, Unwin, RD & Myers, JE 2013, 'A Label-free Selected Reaction Monitoring Workflow Identifies a Subset of Pregnancy Specific Glycoproteins as Potential Predictive Markers of Early-onset Pre-eclampsia', Molecular and Cellular Proteomics, vol. 12, no. 11, pp. 3148-3159. https://doi.org/10.1074/mcp.M112.026872

APA

Vancouver

Author

Blankley, Richard T. ; Fisher, Christal ; Westwood, Melissa ; North, Robyn ; Baker, Philip ; Walker, Michael ; Williamson, Andrew ; Whetton, Anthony D. ; Lin, Wanchang ; McCowan, Lesley ; Roberts, Claire T. ; Cooper, Garth J S ; Unwin, Richard D. ; Myers, Jenny E. / A Label-free Selected Reaction Monitoring Workflow Identifies a Subset of Pregnancy Specific Glycoproteins as Potential Predictive Markers of Early-onset Pre-eclampsia. In: Molecular and Cellular Proteomics. 2013 ; Vol. 12, No. 11. pp. 3148-3159.

Bibtex

@article{158a020276fd440db796e4c5654675b3,
title = "A Label-free Selected Reaction Monitoring Workflow Identifies a Subset of Pregnancy Specific Glycoproteins as Potential Predictive Markers of Early-onset Pre-eclampsia",
abstract = "Pre-eclampsia (PE) is a serious complication of pregnancy with potentially life threatening consequences for both mother and baby. Presently there is no test with the required performance to predict which healthy first-time mothers will go on to develop PE. The high specificity, sensitivity, and multiplexed nature of selected reaction monitoring holds great potential as a tool for the verification and validation of putative candidate biomarkersfor disease states. Realization of this potential involves establishing a high throughput, cost effective, reproducible sample preparation workflow. We have developed a semiautomated HPLC-based sample preparation workflow before a label-free selected reaction monitoring approach. This workflow has been applied to the search for novel predictive biomarkers for PE. To discover novel candidate biomarkers for PE, we used isobaric tagging to identify several potential biomarker proteins in plasma obtained at 15 weeks gestation from nulliparous women who later developed PE compared with pregnant women who remained healthy. Such a study generates a number of {"}candidate{"} biomarkers that require further testing in larger patient cohorts. As proof-of-principle, two of these proteins were taken forward for verification in a 100 women (58 PE, 42 controls) using label-free SRM. We obtained reproducible protein quantitation across the 100 samples and demonstrated significant changes in protein levels, even with as little as 20{\%} change in protein concentration. The SRM data correlated with a commercial ELISA, suggesting that this is a robust workflow suitable for rapid, affordable, label-free verification of which candidate biomarkers should be taken forward for thorough investigation. A subset of pregnancyspecific glycoproteins (PSGs) had value as novel predictive markers for PE. {\circledC} 2013 by The American Society for Biochemistry and Molecular Biology, Inc.",
keywords = "Dementia, human brain, oxidative stress, metabolic stress, type-2 diabetes, copper, experimental therapeutics",
author = "Blankley, {Richard T.} and Christal Fisher and Melissa Westwood and Robyn North and Philip Baker and Michael Walker and Andrew Williamson and Whetton, {Anthony D.} and Wanchang Lin and Lesley McCowan and Roberts, {Claire T.} and Cooper, {Garth J S} and Unwin, {Richard D.} and Myers, {Jenny E.}",
note = ", Cancer Research UK, United Kingdom",
year = "2013",
month = "11",
doi = "10.1074/mcp.M112.026872",
language = "English",
volume = "12",
pages = "3148--3159",
journal = "Molecular and Cellular Proteomics",
issn = "1535-9476",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "11",

}

RIS

TY - JOUR

T1 - A Label-free Selected Reaction Monitoring Workflow Identifies a Subset of Pregnancy Specific Glycoproteins as Potential Predictive Markers of Early-onset Pre-eclampsia

AU - Blankley, Richard T.

AU - Fisher, Christal

AU - Westwood, Melissa

AU - North, Robyn

AU - Baker, Philip

AU - Walker, Michael

AU - Williamson, Andrew

AU - Whetton, Anthony D.

AU - Lin, Wanchang

AU - McCowan, Lesley

AU - Roberts, Claire T.

AU - Cooper, Garth J S

AU - Unwin, Richard D.

AU - Myers, Jenny E.

N1 - , Cancer Research UK, United Kingdom

PY - 2013/11

Y1 - 2013/11

N2 - Pre-eclampsia (PE) is a serious complication of pregnancy with potentially life threatening consequences for both mother and baby. Presently there is no test with the required performance to predict which healthy first-time mothers will go on to develop PE. The high specificity, sensitivity, and multiplexed nature of selected reaction monitoring holds great potential as a tool for the verification and validation of putative candidate biomarkersfor disease states. Realization of this potential involves establishing a high throughput, cost effective, reproducible sample preparation workflow. We have developed a semiautomated HPLC-based sample preparation workflow before a label-free selected reaction monitoring approach. This workflow has been applied to the search for novel predictive biomarkers for PE. To discover novel candidate biomarkers for PE, we used isobaric tagging to identify several potential biomarker proteins in plasma obtained at 15 weeks gestation from nulliparous women who later developed PE compared with pregnant women who remained healthy. Such a study generates a number of "candidate" biomarkers that require further testing in larger patient cohorts. As proof-of-principle, two of these proteins were taken forward for verification in a 100 women (58 PE, 42 controls) using label-free SRM. We obtained reproducible protein quantitation across the 100 samples and demonstrated significant changes in protein levels, even with as little as 20% change in protein concentration. The SRM data correlated with a commercial ELISA, suggesting that this is a robust workflow suitable for rapid, affordable, label-free verification of which candidate biomarkers should be taken forward for thorough investigation. A subset of pregnancyspecific glycoproteins (PSGs) had value as novel predictive markers for PE. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

AB - Pre-eclampsia (PE) is a serious complication of pregnancy with potentially life threatening consequences for both mother and baby. Presently there is no test with the required performance to predict which healthy first-time mothers will go on to develop PE. The high specificity, sensitivity, and multiplexed nature of selected reaction monitoring holds great potential as a tool for the verification and validation of putative candidate biomarkersfor disease states. Realization of this potential involves establishing a high throughput, cost effective, reproducible sample preparation workflow. We have developed a semiautomated HPLC-based sample preparation workflow before a label-free selected reaction monitoring approach. This workflow has been applied to the search for novel predictive biomarkers for PE. To discover novel candidate biomarkers for PE, we used isobaric tagging to identify several potential biomarker proteins in plasma obtained at 15 weeks gestation from nulliparous women who later developed PE compared with pregnant women who remained healthy. Such a study generates a number of "candidate" biomarkers that require further testing in larger patient cohorts. As proof-of-principle, two of these proteins were taken forward for verification in a 100 women (58 PE, 42 controls) using label-free SRM. We obtained reproducible protein quantitation across the 100 samples and demonstrated significant changes in protein levels, even with as little as 20% change in protein concentration. The SRM data correlated with a commercial ELISA, suggesting that this is a robust workflow suitable for rapid, affordable, label-free verification of which candidate biomarkers should be taken forward for thorough investigation. A subset of pregnancyspecific glycoproteins (PSGs) had value as novel predictive markers for PE. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

KW - Dementia, human brain, oxidative stress, metabolic stress, type-2 diabetes, copper, experimental therapeutics

U2 - 10.1074/mcp.M112.026872

DO - 10.1074/mcp.M112.026872

M3 - Article

VL - 12

SP - 3148

EP - 3159

JO - Molecular and Cellular Proteomics

JF - Molecular and Cellular Proteomics

SN - 1535-9476

IS - 11

ER -