A human folliculoid microsphere assay for exploring epithelial- mesenchymal interactions in the human hair follicleCitation formats

  • External authors:
  • Blanka Havlickova
  • Tamás Bíró
  • Alessandra Mescalchin
  • Miriam Tschirschmann
  • Hans Mollenkopf
  • Albrecht Bettermann
  • Paolo Pertile
  • Roland Lauster
  • Enikö Bodó

Standard

A human folliculoid microsphere assay for exploring epithelial- mesenchymal interactions in the human hair follicle. / Havlickova, Blanka; Bíró, Tamás; Mescalchin, Alessandra; Tschirschmann, Miriam; Mollenkopf, Hans; Bettermann, Albrecht; Pertile, Paolo; Lauster, Roland; Bodó, Enikö; Paus, Ralf.

In: Journal of Investigative Dermatology, Vol. 129, No. 4, 04.2009, p. 972-983.

Research output: Contribution to journalArticle

Harvard

Havlickova, B, Bíró, T, Mescalchin, A, Tschirschmann, M, Mollenkopf, H, Bettermann, A, Pertile, P, Lauster, R, Bodó, E & Paus, R 2009, 'A human folliculoid microsphere assay for exploring epithelial- mesenchymal interactions in the human hair follicle', Journal of Investigative Dermatology, vol. 129, no. 4, pp. 972-983. https://doi.org/10.1038/jid.2008.315

APA

Havlickova, B., Bíró, T., Mescalchin, A., Tschirschmann, M., Mollenkopf, H., Bettermann, A., ... Paus, R. (2009). A human folliculoid microsphere assay for exploring epithelial- mesenchymal interactions in the human hair follicle. Journal of Investigative Dermatology, 129(4), 972-983. https://doi.org/10.1038/jid.2008.315

Vancouver

Havlickova B, Bíró T, Mescalchin A, Tschirschmann M, Mollenkopf H, Bettermann A et al. A human folliculoid microsphere assay for exploring epithelial- mesenchymal interactions in the human hair follicle. Journal of Investigative Dermatology. 2009 Apr;129(4):972-983. https://doi.org/10.1038/jid.2008.315

Author

Havlickova, Blanka ; Bíró, Tamás ; Mescalchin, Alessandra ; Tschirschmann, Miriam ; Mollenkopf, Hans ; Bettermann, Albrecht ; Pertile, Paolo ; Lauster, Roland ; Bodó, Enikö ; Paus, Ralf. / A human folliculoid microsphere assay for exploring epithelial- mesenchymal interactions in the human hair follicle. In: Journal of Investigative Dermatology. 2009 ; Vol. 129, No. 4. pp. 972-983.

Bibtex

@article{c59d5d486ccc42239517ae8b33ffc904,
title = "A human folliculoid microsphere assay for exploring epithelial- mesenchymal interactions in the human hair follicle",
abstract = "The search for more effective drugs for the management of common hair growth disorders remains a top priority, both for clinical dermatology and industry. In this pilot study, we report a pragmatic organotypic assay for basic and applied hair research. The patented technique produces microdroplets, which generate human folliculoid microspheres (HFMs), consisting of human dermal papilla fibroblasts and outer root sheath keratinocytes within an extracellular matrix that simulates elements of the hair follicle mesenchyme. Studying a number of different markers (for example, proliferation, apoptosis, cytokeratin-6, versican), we show that these HFMs, cultured under well-defined conditions, retain several essential epithelial-mesenchymal interactions characteristic for human scalp hair follicle. Selected, recognized hair growth-modulatory agents modulate these parameters in a manner that suggests that HFMs allow the standardized preclinical assessment of test agents on relevant human hair growth markers under substantially simplified in vitro conditions that approximate the in vivo situation. Furthermore, we show by immunohistochemistry, reverse transcriptase-PCR, and DNA microarray techniques that HFMs also offer a useful discovery tool for the identification of target genes and their products for candidate hair drugs. HFM thus represent an instructive modern experimental and screening tool for basic and applied hair research in the human system. {\circledC} 2009 The Society for Investigative Dermatology.",
author = "Blanka Havlickova and Tam{\'a}s B{\'i}r{\'o} and Alessandra Mescalchin and Miriam Tschirschmann and Hans Mollenkopf and Albrecht Bettermann and Paolo Pertile and Roland Lauster and Enik{\"o} Bod{\'o} and Ralf Paus",
year = "2009",
month = "4",
doi = "10.1038/jid.2008.315",
language = "English",
volume = "129",
pages = "972--983",
journal = "The Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Springer Nature",
number = "4",

}

RIS

TY - JOUR

T1 - A human folliculoid microsphere assay for exploring epithelial- mesenchymal interactions in the human hair follicle

AU - Havlickova, Blanka

AU - Bíró, Tamás

AU - Mescalchin, Alessandra

AU - Tschirschmann, Miriam

AU - Mollenkopf, Hans

AU - Bettermann, Albrecht

AU - Pertile, Paolo

AU - Lauster, Roland

AU - Bodó, Enikö

AU - Paus, Ralf

PY - 2009/4

Y1 - 2009/4

N2 - The search for more effective drugs for the management of common hair growth disorders remains a top priority, both for clinical dermatology and industry. In this pilot study, we report a pragmatic organotypic assay for basic and applied hair research. The patented technique produces microdroplets, which generate human folliculoid microspheres (HFMs), consisting of human dermal papilla fibroblasts and outer root sheath keratinocytes within an extracellular matrix that simulates elements of the hair follicle mesenchyme. Studying a number of different markers (for example, proliferation, apoptosis, cytokeratin-6, versican), we show that these HFMs, cultured under well-defined conditions, retain several essential epithelial-mesenchymal interactions characteristic for human scalp hair follicle. Selected, recognized hair growth-modulatory agents modulate these parameters in a manner that suggests that HFMs allow the standardized preclinical assessment of test agents on relevant human hair growth markers under substantially simplified in vitro conditions that approximate the in vivo situation. Furthermore, we show by immunohistochemistry, reverse transcriptase-PCR, and DNA microarray techniques that HFMs also offer a useful discovery tool for the identification of target genes and their products for candidate hair drugs. HFM thus represent an instructive modern experimental and screening tool for basic and applied hair research in the human system. © 2009 The Society for Investigative Dermatology.

AB - The search for more effective drugs for the management of common hair growth disorders remains a top priority, both for clinical dermatology and industry. In this pilot study, we report a pragmatic organotypic assay for basic and applied hair research. The patented technique produces microdroplets, which generate human folliculoid microspheres (HFMs), consisting of human dermal papilla fibroblasts and outer root sheath keratinocytes within an extracellular matrix that simulates elements of the hair follicle mesenchyme. Studying a number of different markers (for example, proliferation, apoptosis, cytokeratin-6, versican), we show that these HFMs, cultured under well-defined conditions, retain several essential epithelial-mesenchymal interactions characteristic for human scalp hair follicle. Selected, recognized hair growth-modulatory agents modulate these parameters in a manner that suggests that HFMs allow the standardized preclinical assessment of test agents on relevant human hair growth markers under substantially simplified in vitro conditions that approximate the in vivo situation. Furthermore, we show by immunohistochemistry, reverse transcriptase-PCR, and DNA microarray techniques that HFMs also offer a useful discovery tool for the identification of target genes and their products for candidate hair drugs. HFM thus represent an instructive modern experimental and screening tool for basic and applied hair research in the human system. © 2009 The Society for Investigative Dermatology.

U2 - 10.1038/jid.2008.315

DO - 10.1038/jid.2008.315

M3 - Article

VL - 129

SP - 972

EP - 983

JO - The Journal of Investigative Dermatology

JF - The Journal of Investigative Dermatology

SN - 0022-202X

IS - 4

ER -