A generic platform for the immobilisation of engineered biocatalysts

Research output: Contribution to journalArticle

  • External authors:
  • Matthew P. Thompson
  • Sasha R. Derrington
  • Rachel S. Heath
  • Joanne L. Porter
  • Juan Mangas-Sanchez
  • Paul N. Devine
  • Matthew D. Truppo

Abstract

The application of biocatalysis in the pharmaceutical industry is rapidly growing as a result of increased access to enzymes that meet the demands of industrial processes. This expansion of activity has led to a corresponding increase in the demand for immobilised enzymes. EziG™ (EnginZyme AB, Sweden) is marketed as a general matrix for enzyme immobilisation on controlled porosity glass. In this work we identified criteria for a “general” enzyme immobilisation technology in the context of the requirements of the pharmaceutical industry. We subsequently evaluated EziG™ for generality in a series of case studies for the application of immobilised biocatalysts. In this study we have focussed on the challenges facing both academic and industrial applications such as enzyme stability, multistep reactions and reactions in continuous flow.

Bibliographical metadata

Original languageEnglish
Pages (from-to)327-334
Number of pages8
JournalTetrahedron
Volume75
Issue number3
Early online date6 Dec 2018
DOIs
Publication statusPublished - 18 Jan 2019