A gel-free quantitative proteomics analysis of factors released from hypoxic-conditioned placentae

Research output: Contribution to journalArticle

  • External authors:
  • Richard T. Blankley
  • Nicola J. Robinson
  • Simon J. Gaskell
  • Philip N. Baker

Abstract

Characterizing the protein factors released from placentae during pathogenesis remains a key objective toward understanding preeclampsia and related pregnancy disorders. Gel-free proteomics technologies applied to placental explant-conditioned media offers the potential of identifying these factors. Relative quantification mass spectrometry using isobaric tagging for relative and absolute quantification (iTRAQ) labeling was employed to compare the "secretome" between healthy term placental tissue cultured under both normoxic and hypoxic oxygen tensions. Of the 499 proteins identified, 45 were differentially expressed (P

Bibliographical metadata

Original languageEnglish
Pages (from-to)247-257
Number of pages10
JournalREPRODUCTIVE SCIENCES
Volume17
Issue number3
DOIs
Publication statusPublished - Mar 2010