A gel-free quantitative proteomics analysis of factors released from hypoxic-conditioned placentae

Research output: Contribution to journalArticle

  • External authors:
  • Richard T. Blankley
  • Nicola J. Robinson
  • Simon J. Gaskell
  • Philip N. Baker


Characterizing the protein factors released from placentae during pathogenesis remains a key objective toward understanding preeclampsia and related pregnancy disorders. Gel-free proteomics technologies applied to placental explant-conditioned media offers the potential of identifying these factors. Relative quantification mass spectrometry using isobaric tagging for relative and absolute quantification (iTRAQ) labeling was employed to compare the "secretome" between healthy term placental tissue cultured under both normoxic and hypoxic oxygen tensions. Of the 499 proteins identified, 45 were differentially expressed (P

Bibliographical metadata

Original languageEnglish
Pages (from-to)247-257
Number of pages10
Issue number3
Publication statusPublished - Mar 2010