A correlation between residual radiation-induced DNA double-strand breaks in cultured fibroblasts and late radiotherapy reactions in breast cancer patientsCitation formats

  • External authors:
  • Anne E. Kiltie
  • Anderson J. Ryan
  • Ric Swindell
  • James B P Barber
  • Brian Magee
  • Jolyon H. Hendry

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A correlation between residual radiation-induced DNA double-strand breaks in cultured fibroblasts and late radiotherapy reactions in breast cancer patients. / Kiltie, Anne E.; Ryan, Anderson J.; Swindell, Ric; Barber, James B P; West, Catharine M L; Magee, Brian; Hendry, Jolyon H.

In: Radiotherapy and Oncology, Vol. 51, No. 1, 01.04.1999, p. 55-65.

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Kiltie, Anne E. ; Ryan, Anderson J. ; Swindell, Ric ; Barber, James B P ; West, Catharine M L ; Magee, Brian ; Hendry, Jolyon H. / A correlation between residual radiation-induced DNA double-strand breaks in cultured fibroblasts and late radiotherapy reactions in breast cancer patients. In: Radiotherapy and Oncology. 1999 ; Vol. 51, No. 1. pp. 55-65.

Bibtex

@article{0f33297b118e4f5da1ab6a37ba8c1e4c,
title = "A correlation between residual radiation-induced DNA double-strand breaks in cultured fibroblasts and late radiotherapy reactions in breast cancer patients",
abstract = "Background and purpose: Prediction of late normal tissue reactions to radiotherapy would permit tailoring of dosage to each patient. Measurement of residual DNA double strand breaks using pulsed field gel electrophoresis (PFGE) shows promise in this field. The aim of this study was to test the predictive potential of PFGE in a group of retrospectively studied breast cancer patients. Materials and methods: Thirty nine patients, treated uniformly for breast cancer 9-15 years previously, with excision of the tumour and radiotherapy to the breast and drainage areas, were assessed clinically using the LENT SOMA scale, and a 5-mm punch biopsy taken from the buttock. Fibroblast cell strains were established and used to study residual DNA double strand breaks, using PFGE. Results: There were significant correlations between the DNA assay results and the fibrosis score (r(s) = 0.46; P = 0.003), the combined fibrosis and retraction score (r(s) = 0.45, P = 0.004) and the overall LENT Score (r(s)=0.43; P = 0.006). Using polychotomous logistic regression, the fibroblast DNA assay result was an independent prognostic factor for fibrosis severity. Conclusions: There is a relationship between residual radiation-induced DNA damage in fibroblasts and the severity of the late normal tissue damage seen in the patients from whom the cells were cultured.",
keywords = "DNA damage, Fibroblasts, Normal tissues, Predictive assays, Radiosensitivity, Radiotherapy",
author = "Kiltie, {Anne E.} and Ryan, {Anderson J.} and Ric Swindell and Barber, {James B P} and West, {Catharine M L} and Brian Magee and Hendry, {Jolyon H.}",
year = "1999",
month = apr,
day = "1",
doi = "10.1016/S0167-8140(99)00030-4",
language = "English",
volume = "51",
pages = "55--65",
journal = "Radiotherapy & Oncology",
issn = "0167-8140",
publisher = "Elsevier BV",
number = "1",

}

RIS

TY - JOUR

T1 - A correlation between residual radiation-induced DNA double-strand breaks in cultured fibroblasts and late radiotherapy reactions in breast cancer patients

AU - Kiltie, Anne E.

AU - Ryan, Anderson J.

AU - Swindell, Ric

AU - Barber, James B P

AU - West, Catharine M L

AU - Magee, Brian

AU - Hendry, Jolyon H.

PY - 1999/4/1

Y1 - 1999/4/1

N2 - Background and purpose: Prediction of late normal tissue reactions to radiotherapy would permit tailoring of dosage to each patient. Measurement of residual DNA double strand breaks using pulsed field gel electrophoresis (PFGE) shows promise in this field. The aim of this study was to test the predictive potential of PFGE in a group of retrospectively studied breast cancer patients. Materials and methods: Thirty nine patients, treated uniformly for breast cancer 9-15 years previously, with excision of the tumour and radiotherapy to the breast and drainage areas, were assessed clinically using the LENT SOMA scale, and a 5-mm punch biopsy taken from the buttock. Fibroblast cell strains were established and used to study residual DNA double strand breaks, using PFGE. Results: There were significant correlations between the DNA assay results and the fibrosis score (r(s) = 0.46; P = 0.003), the combined fibrosis and retraction score (r(s) = 0.45, P = 0.004) and the overall LENT Score (r(s)=0.43; P = 0.006). Using polychotomous logistic regression, the fibroblast DNA assay result was an independent prognostic factor for fibrosis severity. Conclusions: There is a relationship between residual radiation-induced DNA damage in fibroblasts and the severity of the late normal tissue damage seen in the patients from whom the cells were cultured.

AB - Background and purpose: Prediction of late normal tissue reactions to radiotherapy would permit tailoring of dosage to each patient. Measurement of residual DNA double strand breaks using pulsed field gel electrophoresis (PFGE) shows promise in this field. The aim of this study was to test the predictive potential of PFGE in a group of retrospectively studied breast cancer patients. Materials and methods: Thirty nine patients, treated uniformly for breast cancer 9-15 years previously, with excision of the tumour and radiotherapy to the breast and drainage areas, were assessed clinically using the LENT SOMA scale, and a 5-mm punch biopsy taken from the buttock. Fibroblast cell strains were established and used to study residual DNA double strand breaks, using PFGE. Results: There were significant correlations between the DNA assay results and the fibrosis score (r(s) = 0.46; P = 0.003), the combined fibrosis and retraction score (r(s) = 0.45, P = 0.004) and the overall LENT Score (r(s)=0.43; P = 0.006). Using polychotomous logistic regression, the fibroblast DNA assay result was an independent prognostic factor for fibrosis severity. Conclusions: There is a relationship between residual radiation-induced DNA damage in fibroblasts and the severity of the late normal tissue damage seen in the patients from whom the cells were cultured.

KW - DNA damage

KW - Fibroblasts

KW - Normal tissues

KW - Predictive assays

KW - Radiosensitivity

KW - Radiotherapy

U2 - 10.1016/S0167-8140(99)00030-4

DO - 10.1016/S0167-8140(99)00030-4

M3 - Article

VL - 51

SP - 55

EP - 65

JO - Radiotherapy & Oncology

JF - Radiotherapy & Oncology

SN - 0167-8140

IS - 1

ER -