A biocatalytic cascade for the amination of unfunctionalised cycloalkanes

Research output: Contribution to journalArticle


Here we describe a one-pot, three-enzyme, cascade involving a cytochrome P450 monooxygenase, an alcohol dehydrogenase and a reductive aminase for the synthesis of secondary amines from cycloalkanes. Amine product concentrations of up to 19.6 mM were achieved. The preparative scale amination of cyclohexane was also demonstrated with a space-time yield of 2 g L-1 d-1.

Bibliographical metadata

Original languageEnglish
Pages (from-to)9790-9793
Number of pages4
JournalOrganic and Biomolecular Chemistry
Issue number46
Early online date10 Nov 2017
Publication statusPublished - 14 Dec 2017