A Bifunctional Spin-Label for Ligand Recognition on Surfaces

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In situ monitoring of biomolecular recognition, especially at surfaces, still presents a significant technical challenge. Electron paramagnetic resonance (EPR) of biomolecules spin-labelled with nitroxides can offer uniquely sensitive and selective insights into these processes, but new spin-labelling strategies are needed. We report the synthesis and study of a bromoacrylaldehyde spin-label (BASL), which features two attachment points with orthogonal reactivity. We have created the first examples of mannose and biotin ligands coupled to aqueous carboxy-functionalized gold nanoparticles through a spin-label. EPR spectra were obtained for the spin-labelled ligands both free in solution and attached to nanoparticles. The labels were recognised by the mannose-binding lectin, Con A, and the biotin-binding protein avidin-peroxidase. Binding gave quantifiable changes in the EPR spectra from which binding profiles could be obtained that reflect the strength of binding in each case.

Bibliographical metadata

Original languageEnglish
JournalAngewandte Chemie
Early online date1 Jun 2017
Publication statusPublished - 2017

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