3D organotypic spinal culturesCitation formats

  • External authors:
  • Mattia Musto
  • Rossana Rauti
  • Artur Filipe Rodrigues
  • Elena Bonechi
  • Clara Ballerini
  • Laura Ballerini

Standard

3D organotypic spinal cultures : Exploring neuron and neuroglia responses upon prolonged exposure to graphene oxide. / Musto, Mattia; Rauti, Rossana; Rodrigues, Artur Filipe; Bonechi, Elena; Ballerini, Clara; Kostarelos, Kostas; Ballerini, Laura.

In: Frontiers in Systems Neuroscience, Vol. 13, 1, 24.01.2019.

Research output: Contribution to journalArticle

Harvard

Musto, M, Rauti, R, Rodrigues, AF, Bonechi, E, Ballerini, C, Kostarelos, K & Ballerini, L 2019, '3D organotypic spinal cultures: Exploring neuron and neuroglia responses upon prolonged exposure to graphene oxide', Frontiers in Systems Neuroscience, vol. 13, 1. https://doi.org/10.3389/fnsys.2019.00001

APA

Musto, M., Rauti, R., Rodrigues, A. F., Bonechi, E., Ballerini, C., Kostarelos, K., & Ballerini, L. (2019). 3D organotypic spinal cultures: Exploring neuron and neuroglia responses upon prolonged exposure to graphene oxide. Frontiers in Systems Neuroscience, 13, [1]. https://doi.org/10.3389/fnsys.2019.00001

Vancouver

Musto M, Rauti R, Rodrigues AF, Bonechi E, Ballerini C, Kostarelos K et al. 3D organotypic spinal cultures: Exploring neuron and neuroglia responses upon prolonged exposure to graphene oxide. Frontiers in Systems Neuroscience. 2019 Jan 24;13. 1. https://doi.org/10.3389/fnsys.2019.00001

Author

Musto, Mattia ; Rauti, Rossana ; Rodrigues, Artur Filipe ; Bonechi, Elena ; Ballerini, Clara ; Kostarelos, Kostas ; Ballerini, Laura. / 3D organotypic spinal cultures : Exploring neuron and neuroglia responses upon prolonged exposure to graphene oxide. In: Frontiers in Systems Neuroscience. 2019 ; Vol. 13.

Bibtex

@article{4129d8c0a4d4417896a84c0d07e54c5e,
title = "3D organotypic spinal cultures: Exploring neuron and neuroglia responses upon prolonged exposure to graphene oxide",
abstract = "Graphene-based nanomaterials are increasingly engineered as components of biosensors, interfaces or drug delivery platforms in neuro-repair strategies. In these developments, the mostly used derivative of graphene is graphene oxide (GO). To tailor the safe development of GO nanosheets, we need to model in vitro tissue responses, and in particular the reactivity of microglia, a sub-population of neuroglia that acts as the first active immune response, when challenged by GO. Here, we investigated central nervous system (CNS) tissue reactivity upon long-term exposure to GO nanosheets in 3D culture models. We used the mouse organotypic spinal cord cultures, ideally suited for studying long-term interference with cues delivered at controlled times and concentrations. In cultured spinal segments, the normal presence, distribution and maturation of anatomically distinct classes of neurons and resident neuroglial cells are preserved. Organotypic explants were developed for 2 weeks embedded in fibrin glue alone or presenting GO nanosheets at 10, 25 and 50 μg/mL. We addressed the impact of such treatments on premotor synaptic activity monitored by patch clamp recordings of ventral interneurons. We investigated by immunofluorescence and confocal microscopy the accompanying glial responses to GO exposure, focusing on resident microglia, tested in organotypic spinal slices and in isolated neuroglia cultures. Our results suggest that microglia reactivity to accumulation of GO flakes, maybe due to active phagocytosis, may trim down synaptic activity, although in the absence of an effective activation of inflammatory response and in the absence of neuronal cell death.",
keywords = "Graphene oxide, Microglia, Microvesicles, Organotypic cultures, Patch-clamp",
author = "Mattia Musto and Rossana Rauti and Rodrigues, {Artur Filipe} and Elena Bonechi and Clara Ballerini and Kostas Kostarelos and Laura Ballerini",
year = "2019",
month = "1",
day = "24",
doi = "10.3389/fnsys.2019.00001",
language = "English",
volume = "13",
journal = "Frontiers in Systems Neuroscience",
issn = "1662-5137",
publisher = "Frontiers Research Foundation",

}

RIS

TY - JOUR

T1 - 3D organotypic spinal cultures

T2 - Exploring neuron and neuroglia responses upon prolonged exposure to graphene oxide

AU - Musto, Mattia

AU - Rauti, Rossana

AU - Rodrigues, Artur Filipe

AU - Bonechi, Elena

AU - Ballerini, Clara

AU - Kostarelos, Kostas

AU - Ballerini, Laura

PY - 2019/1/24

Y1 - 2019/1/24

N2 - Graphene-based nanomaterials are increasingly engineered as components of biosensors, interfaces or drug delivery platforms in neuro-repair strategies. In these developments, the mostly used derivative of graphene is graphene oxide (GO). To tailor the safe development of GO nanosheets, we need to model in vitro tissue responses, and in particular the reactivity of microglia, a sub-population of neuroglia that acts as the first active immune response, when challenged by GO. Here, we investigated central nervous system (CNS) tissue reactivity upon long-term exposure to GO nanosheets in 3D culture models. We used the mouse organotypic spinal cord cultures, ideally suited for studying long-term interference with cues delivered at controlled times and concentrations. In cultured spinal segments, the normal presence, distribution and maturation of anatomically distinct classes of neurons and resident neuroglial cells are preserved. Organotypic explants were developed for 2 weeks embedded in fibrin glue alone or presenting GO nanosheets at 10, 25 and 50 μg/mL. We addressed the impact of such treatments on premotor synaptic activity monitored by patch clamp recordings of ventral interneurons. We investigated by immunofluorescence and confocal microscopy the accompanying glial responses to GO exposure, focusing on resident microglia, tested in organotypic spinal slices and in isolated neuroglia cultures. Our results suggest that microglia reactivity to accumulation of GO flakes, maybe due to active phagocytosis, may trim down synaptic activity, although in the absence of an effective activation of inflammatory response and in the absence of neuronal cell death.

AB - Graphene-based nanomaterials are increasingly engineered as components of biosensors, interfaces or drug delivery platforms in neuro-repair strategies. In these developments, the mostly used derivative of graphene is graphene oxide (GO). To tailor the safe development of GO nanosheets, we need to model in vitro tissue responses, and in particular the reactivity of microglia, a sub-population of neuroglia that acts as the first active immune response, when challenged by GO. Here, we investigated central nervous system (CNS) tissue reactivity upon long-term exposure to GO nanosheets in 3D culture models. We used the mouse organotypic spinal cord cultures, ideally suited for studying long-term interference with cues delivered at controlled times and concentrations. In cultured spinal segments, the normal presence, distribution and maturation of anatomically distinct classes of neurons and resident neuroglial cells are preserved. Organotypic explants were developed for 2 weeks embedded in fibrin glue alone or presenting GO nanosheets at 10, 25 and 50 μg/mL. We addressed the impact of such treatments on premotor synaptic activity monitored by patch clamp recordings of ventral interneurons. We investigated by immunofluorescence and confocal microscopy the accompanying glial responses to GO exposure, focusing on resident microglia, tested in organotypic spinal slices and in isolated neuroglia cultures. Our results suggest that microglia reactivity to accumulation of GO flakes, maybe due to active phagocytosis, may trim down synaptic activity, although in the absence of an effective activation of inflammatory response and in the absence of neuronal cell death.

KW - Graphene oxide

KW - Microglia

KW - Microvesicles

KW - Organotypic cultures

KW - Patch-clamp

U2 - 10.3389/fnsys.2019.00001

DO - 10.3389/fnsys.2019.00001

M3 - Article

VL - 13

JO - Frontiers in Systems Neuroscience

JF - Frontiers in Systems Neuroscience

SN - 1662-5137

M1 - 1

ER -